The use of US-origin or USDA-grade allows researchers to send their cells, or the products of their cells, to collaborators in other countries with strict import regulations. Serum, as a biological material, represents an undefined mixture of components in which the fetal bovine serum composition varies from one lot to the other.
Some cell types are sensitive to the variations in serum performance. Customers are encouraged to evaluate serum samples with their own culture system and cells while we reserve the quantities of the specific lots until customer testing is completed. To learn more, please feel free to email us at custom-services bioind.
Cookie Policy This website uses cookies to ensure proper functionality of the shopping cart and checkout progress. Accept Decline. Contact us. Biological Industries. Our History. Search: Enter keyword or catalog number. Introduction to Fetal Bovine Serum Class. Providing researchers with quality and transparency of FBS Serum is commonly used as a supplement to basal growth medium in cell culture.
Quality Control Each lot of FBS is tested to confirm that the serum meets the written specifications. Mycoplasma contamination: according to the CFR, title 9, part culture method. Endotoxins: test is performed using kinetic colorimetric method.
Biological performance Cell growth tests are designed to check the efficacy of the FBS in promoting cell growth. Hybridoma cells: cloning efficiency testing. Quality assurance - CE mark Biological Industries FBS production process is carried out under controlled conditions in a controlled environment. We have sera qualified for specialty research and specific assays, including stem cell research, immunoassays, antibodies, and more.
This energy is transferred from the photons to the products, and is responsible for organism inactivation by ionization of nucleic acid bonds. The irradiation of serum is intended to provide complete assurance of viral inactivation. An extensive validation study has been performed to validate the irradiation process using FBS spiked with several viruses. We have demonstrated that properties and cell culture performance of FBS are not altered by gamma irradiation exposure up to 3.
While whole serum is permissible for routine purposes, studies involving nutritional parameters or incorporation of labeled material require that the constituent under study be removed from the serum. The most commonly used method for removal of these constituents is dialysis of whole serum. For dialysis by diafiltration, serum is circulated through a hollow-fiber by the concentration method. The filtrate however, is replaced by the addition of physiological saline to the serum.
Heat inactivation is the method of choice to destroy complement, and to ensure that the cells will not be lysed by antibody binding. The stem cells can be maintained in vitro for extended periods without loss of their capacity to differentiate to all cell lineages when reimplanted back into a blastocyst.
This type of standardization is even more critical in regulated laboratories and using a defined medium allows greater control over your cell culture and your experiments. Another potential issue with serum is viral or bacterial contamination.
Again, lot-to-lot variability is in play here, and heat inactivation may not wholly neutralize microbial pathogens. Adding contaminated serum to your culture will affect the health and growth of your cells and render them unusable for experiments. Maybe you are convinced that going serum-free is the best approach for your cell culture. But like many things in science, it comes with trade-offs, and one of them is finding exactly the right mix of growth factors to support your culture.
The needs of different established cell lines are varied and complex. Optimal culture conditions can even change between different passages of cells! Throw in primary cultures or culture in suspension and you will have quite the task in front of you. Fortunately, you are not alone, and there are many expert sources online, to guide you. Besides the difficulty in getting the serum-free conditions just right, other adverse effects of going serum-free include slower cell growth and cell clumping when passaging.
Continued optimization of culture conditions can help with the reduced rate of growth, and gentle repeated pipetting of cell clumps should be enough to disperse them. Note that some cell types can be cultured in the complete absence of serum, while others will need other factors added to replace the role of serum in the media. Several serum alternatives are available to purchase that you can try as an alternative to completely removing serum, however, some cell types will have more unique needs that require adding specific factors to the media.
The FCS-free Database provides details of additives noted in the literature for specific cell lines to be cultured without foetal calf serum.
Register now. Login I forgot my password. There are no items in your shopping cart. Why is Fetal bovine serum used? What causes cell culture problems? The drivers behind these undesired outcomes are: The concentration of serum proteins is generally much higher than the protein produced by mammalian cells in vivo. The abundantly available nutrients in FBS overfeed the cells, they become too large, proliferate faster, and the morphology is atypical.
Serum proteins are a significant contaminant and may unwantedly interact with your component of interest. Serum components may also bind, degrade or otherwise interact with substances added to the medium.
These factors may influence the observed effect and lead to false conclusions. If the target protein is related to a serum protein, you can't separate the two easily. On top of that, harvesting serum from unborn calves in slaughterhouses poses ethical concerns. Serum contamination with viruses, bacteria, fungi, or BSE has been a concern for many years. How do you recognize it? Our advice to solve these cell culture media problems Medium without the need to add FBS or other serum is the ultimate solution.
For research, serum-free media also benefit from eliminating the risk of infectious agents while supporting the growth of a wide range of cell types.
Serum-free medium has fewer possible interfering factors due to low nutrient levels. Defining the components makes it possible to find root causes when problems occur in the research setting.
In serum-free media , the protein concentration is low, resulting in low levels of contaminants.
0コメント